Short answer. Yes. Donations given to children are charged to their legitime, so the lot counts as an advance on your brother's share rather than a gift on top of it. If its value exceeds what he was due, the excess is reduced under the Civil Code's rules.
What the law says
Donations given to children shall be charged to their legitime.
Civil Code, Article 909 — Charging Donations To Shares. Read the full provision →
A gift to a child is an advance, not a bonus
The default the Civil Code sets is equality among children, and this article is how it enforces that. A lot handed to one child during the parent's lifetime is treated as part of what that child was going to receive anyway. So when the estate is divided, the value of the lot is set against your brother's legitime and he takes correspondingly less from what remains. The practical effect is that the gift changes the timing of his inheritance, not its size.
What happens if the lot was worth more than his share
Sometimes the gift swallows more than the child was entitled to. The article deals with that too: Insofar as they may be inofficious or may exceed the disposable portion, they shall be reduced according to the rules established by this Code. Reduction means the excess is brought back into the estate so the other compulsory heirs can be made whole. It does not mean the whole donation is undone — only the part that trespasses on shares the law reserved for someone else.
How the lot is valued is the fight
Most family arguments about a long-ago transfer are really arguments about numbers. The relevant figure is the value of the property when the donation was made, not what the land is worth today, so a lot given cheaply decades ago is charged at its then value even if it has appreciated many times over. That can feel unfair to the other children, but it is the rule the Code applies, and it makes the deed of donation and its date the most important document in the file.
Check first whether it really was a donation
Before any of this applies, establish what the transfer actually was. A deed of absolute sale for a real price is not a donation, though a sale at a nominal price to a child often is one in substance. Get a certified copy of the instrument, the title history, and any proof that money did or did not change hands. Raise the advance during settlement and before signing a partition — an extrajudicial settlement that ignores the gift is very hard to reopen afterwards. Ask for the deed itself, not a description of it: the date and the wording of any waiver are what decide the argument.