Short answer. Yes. The marital disqualification rule bars spouses from testifying against each other during marriage, but it expressly excepts a criminal case for a crime committed by one spouse against the other spouse's direct descendants — your child qualifies, so his consent is not required for you to testify.
What the law says
except in a civil case by one against the other, or in a criminal case for a crime committed by one against the other or the latter's direct descendants or ascendants
Rule 130, Section 23 — Disqualification by reason of marriage. Read the full provision →
The general rule you are asking about
The default rule is protective of marriage: during their marriage, the husband or the wife cannot testify against the other without the consent of the affected spouse. That default exists to preserve marital harmony and to spare a spouse from being compelled to give testimony that could harm their own husband or wife. Standing alone, it would block you from testifying against him. The rule binds both spouses equally and applies regardless of which one is asked to testify against the other.
The exception that applies to your situation
The rule carries a built-in exception, and it covers exactly this scenario: a criminal case for a crime committed by one spouse against the other or the latter's direct descendants or ascendants. Your child, as your descendant, falls squarely within that phrase. Because the crime was committed against your child rather than only against you personally, the marital disqualification does not stand in the way of your testimony.
Why consent is not the deciding factor here
Under the general rule, the affected spouse's consent can unlock testimony that would otherwise be barred. But once a case falls within the exception — a crime against a spouse's own descendant — the rule does not condition your testimony on his consent at all. The exception removes the disqualification itself for that kind of case, rather than merely offering a way around it that still depends on his agreement.
The same exception covers ascendants too
The wording that opens the door for your child does the same for a crime committed against a spouse's ascendants — parents or grandparents, for instance. The rule is built around the idea that a spouse should not be shielded by marriage when the victim is someone in the other spouse's own direct family line, whether that victim is a child, grandchild, parent, or grandparent — the family relationship to the testifying spouse is what triggers the exception, not the particular offense charged. What the exception does not do is remove the disqualification for crimes against people outside that direct line, such as a spouse's sibling or friend.